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I can't think why not. Back when I rooted phones I always used a Mac.

Looking at this recipe on XDA I can't see anything Windows-specific (though the author uses Windows). The only software it needs is Fastboot, which is actually easier on a Mac than Windows (you can just download a fastboot executable, no need to install a whole set of SDK tools, and you don't need to faff installing USB drivers). The rest does not depend on what OS you are using, other than that you'd use a MacOS terminal rather than a Windows command line.
 
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